Establishment of a New Epstein - Barr Virus - Immortalized Cell Line From Chronic Lymphocytic Leukemia With Trisomy of Chromosome 12
نویسندگان
چکیده
C HRONIC LYMPHOCYTIC LEUKEMIA (CLL) B cells are, in general, highly refractory to transformation by Epstein-Barr virus (EBV),’ and therefore, relatively few EBV-immortalized cell lines originating from CLL cells have been described.’4 In this paper we report the establishment of a novel EBV-transformed B cell line from the leukemic cells of a CLL patient that expressed surface u immunoglobulin. A distinctive feature of these leukemic cells, which makes this cell line unique, is that their surface 1gM reacted with a sheep RBC (SRBC) antigen. Specific binding of SRBC was maintained by the EBV-transformed cells, which indicated their leukemic origin. This was futher confirmed by karyotypic analysis showing that the immortalized cells carried trisomy of chromosome I 2, a cytogenetic abnormality typical of CLL of the B cell type.5
منابع مشابه
Establishment of a new Epstein-Barr virus-immortalized cell line from chronic lymphocytic leukemia with trisomy of chromosome 12 that produces monoclonal IgM against a sheep RBC antigen.
Leukemia cells from a patient with chronic lymphocytic leukemia (CLL) were found to bind sheep RBC (SRBC) through their monoclonal surface IgM. A lymphoblastoid cell line was obtained by immortalization of leukemic cells with Epstein-Barr virus (EBV). Cultured leukemic cells were found to have a supernumerary chromosome 12, an abnormality typical of CLL of the B cell type. To our knowledge, thi...
متن کاملEstablishment of a New Epstein - Barr Virus - Immortalized Cell Line From Chronic
C HRONIC LYMPHOCYTIC LEUKEMIA (CLL) B cells are, in general, highly refractory to transformation by Epstein-Barr virus (EBV),’ and therefore, relatively few EBV-immortalized cell lines originating from CLL cells have been described.’4 In this paper we report the establishment of a novel EBV-transformed B cell line from the leukemic cells of a CLL patient that expressed surface u immunoglobulin....
متن کاملCharacterization of a New Chronic Lymphocytic Leukemia Cell Line for Mechanistic In Vitro and In Vivo Studies Relevant to Disease
Studies of chronic lymphocytic leukemia (CLL) have yielded substantial progress, however a lack of immortalized cell lines representative of the primary disease has hampered a full understanding of disease pathogenesis and development of new treatments. Here we describe a novel CLL cell line (OSU-CLL) generated by EBV transformation, which displays a similar cytogenetic and immunophenotype obse...
متن کاملVR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
BACKGROUND small B-cell neoplasms can show plasmacytic differentiation and may potentially progress to aggressive lymphoma (DLBCL). Epstein-Barr virus (EBV) infection may cause the transformation of malignant cells in vitro. DESIGN AND METHOD we established VR09 cell line with plasmacytic differentiation, obtained from a case of atypical, non-CLL B-cell chronic lymphoproliferative disease wit...
متن کاملEvaluation of Changes in NFKB Gene Expression Following Epstein-Barr Virus and its Participation in the Half-Life of Patients With Acute Epstein-Barr Positive Lymphoblastic Leukemia
Aim: Leukemia targets are one of the most common childhood malignancies. Epstein-Barr virus is a tumorigenic virus of the herpes family of viruses and causes primary infection in young children. The aim of this study was to evaluate the increase in NFKB expression following EBV virus and its contribution to the half-life of EBV-positive acute lymphoblastic leukemia patients. Materials and Meth...
متن کامل